Identification of Genetic Polymorphism in Resistin (RETN) Gene and its Influence on Reproduction and Production Traits of Indian Dairy Cattle Pandey Vijay1,*, Nigam Rajesh1, Sharma Deepak2, Singh Satyendra Pal2, Tiwari Madhu2 1Department of Biochemistry, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashuchikitsa Vigyan Vishwavidyalya evam Go Anusandhan Sansthan (DUVASU), Mathura, India 2Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashuchikitsa Vigyan Vishwavidyalya evam Go Anusandhan Sansthan (DUVASU), Mathura, India *Corresponding author: V Pandey, Email: drvijaypandey@gmail.com
Online published on 7 February, 2019. Abstract The present study was executed to elucidate the genetic polymorphism in resistin (RETN) gene and its association with reproduction and production traits in Sahiwal cattle. A fragment of exon-2 region of RETN gene from genomic DNA of Sahiwal cattle was amplified by PCR that resulted in amplicon of 338 bp. The obtained amplicon was subjected to Single Stranded Confirmation Polymorphism (SSCP) technique for identification of genetic polymorphism which revealed two genotypes, homozygous AA and heterozygous AB genotypes. In the studied population of Sahiwal cattle AA was most frequent genotype (67.24%) than AB genotype (32.76%). The frequency of A and B alleles were 0.836 and 0.164, respectively. The association study of RETN/SSCP assay revealed significant influence of genotypes on birth weight (BW), dry period (DP) and lactation period (LP). The RETN/SSCP polymorphism revealed higher BW in AA than AB genotype. The study showed significantly longer DP in AB than AA genotype in second lactation while significantly longer LP was observed in AA compared to AB genotypes in first lactation of Sahiwal cows. In conclusion, PCR-SSCP assay was found to be capable of detecting genetic polymorphism in exon-2 region of RETN gene and its association with reproduction and production traits suggests that A allele of this gene might serves as candidate genetic marker for selection of Sahiwal cattle with better reproductive and production traits. However, further studies are warranted to discover this genetic polymorphism in another breed and population of cattle. Top Keywords RETN gene, PCR-SSCP, Reproduction, Production, Sahiwal cattle. Top |