Serological, Isolation and Molecular Studies on Brucellosis in an Organized Farm, Jammu and Kashmir, India Singh Maninder1,*, Malik M.A.1, Singh Rajiv2, Singh D.K.3, Doimari Soni4, Tandon Suhasani1, Gupta Jyoti5 1Divsion of Veterinary Public Health and Epidemiology, F.V.Sc & A.H., SKUAST-Jammu, R.S.Pura, Jammu, Jammu and Kashmir, India 2Divsion of Veterinary Medicine, F.V.Sc & A.H., SKUAST-Jammu, R.S.Pura, Jammu, Jammu and Kashmir, India 3Divsion of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India 4Department of Veterinary Public Health, L.C.V.Sc, Assam Agricultural University, Assam, India 5Department of Animal Husbandry, Jammu, Jammu and Kashmir, India *Corresponding author: M Singh; Email: manindersingh2k2@gmail.com
Online published on 3 December, 2019. Abstract The present study was conducted in an organized farm of Jammu region, Jammu and Kashmir state having history of late-term abortions in cattle and buffaloes to elucidate the role of brucellosis in causing abortions on the farm. The farm had 46 animals (23 adults, 15 heifers and 9 calves). The farm was positive in herd test (milk ring test). Serological tests viz., Rose Bengal plate test (RBPT) and serum agglutination test (SAT) were conducted on 30 serum samples and 11 samples were found positive in both tests. Five vaginal swabs from abortion cases were processed for isolation and 3 isolates of Brucella were obtained which on further characterization by biochemical tests, genus-specific PCR and species-specific PCR were identified as B. abortus biovar 1. As brucellosis is an anthropozoonoses, the high prevalence of brucellosis in livestock of farm demands the surveillance of disease in humans working on the farm and necessitates the control of disease on the farm. Top Keywords Brucellosis, isolation, Jammu and Kashmir. Top |