The isolation, culture and identification of skeletal muscle satellite cells from bactrian camel Deng Tianjian1,6, Liu Tuya2, Tuya3, Yang Bin1, Cui Altenwula4, Surlig5, Sharku5, Bayartai5, Demtu Er1 1College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, 010018, China 2Veterinary Bureau of Alxa Left Banner, Bayanhot, 750300, Inner Mongolia Autonomous Region, P. R. China 3Detachment of Alxa league Agriculture and Animal Husbandry Comprehensive Administrative Law Enforcement, Bayanhot, 750306, Inner Mongolia Autonomous region, P. R. China 4Aminal supervision station of Agriculture and Animal Husbandry Integrated Service Centre, Barenbelle Town, Alxa Left Banner, 750308, Inner Mongolia autonomous region, P. R. China 5Agriculture and Animal Husbandry Integrated Service Centre of Yinggen Sumu, Alxa left banner, Inner Mongolia Autonomous Region, P.R. China 6Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Huhhot, 010018, China email: eedmt@imau.edu.cn
Online published on 30 January, 2020. Abstract In this experiment, the skeletal muscle tissue of Bactrian camel was used as sample material. The cells of primary generation were cultured by two-step enzymatic digestion and tissue block culture. The primary skeletal muscle satellite cells were purified by differential adherence methods, and then were determined by growth curve analysis. The advantages of mode of proliferation of cells were that the cells could maintain high activity, comparatively not tend to be contaminated, and gain a long culture period, which is more suitable for the in vitro culture of Bactrian camel satellite cells. When using the method called LASER Confocal imaging, with immunofluorescence labeling, to identify the specific gene in skeletal muscle satellite cells, the PAX7 expression was positive in cell lines. In addition, PCR amplification bands showed that PAX7, MYF5, and Desmin genes were all clearly expressed. After induced culture, satellite cells started the myogenic differentiation process. Through the above methods, comparatively pure Bactrian camel skeletal muscle satellite cells were obtained successfully and passage proces were carried out stably. In conclusion, the in vitro culture system of Bactrian camel skeletal muscle satellite cells was successfully established. Top Keywords Bactrian camel, in vitro culture, skeletal muscle satellite cells. Top |