Year : 2022, Volume : 17, Issue : 1
First page : ( 174) Last page : ( 176)
Print ISSN : 2229-628X. Online ISSN : 2582-2683. Published online : 2022 January 4.
Article DOI : 10.5958/2582-2683.2022.00037.5

Molecular characterization of the pigeon pea cyst nematode, Heteroderacajani Koshy, 1967 from India

Kumar K.Kiran¹²*, Sutar Vivek¹, Davies Keith G.³, Mohan Sharad¹**

¹Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India

²ICAR-Central Citrus Research Institute, Nagpur-440 033, Maharashtra, India

³Department of Biological and Environmental Sciences, University of Hertfordshire, Hatfield, United Kingdom

*Authors for correspondence: kiranag0734@gmail.com

**sharad@iari.res.in

Received:  23  ,  2021; Accepted:  26  October,  2021.

Abstract

Cysts of Heteroderacajani was characterized via molecular analysis using D2-D3 regionof 28S ribosomal DNA (rDNA). Blast N analysis of the amplified fragment in NCBI database revealed that H.cajani IARI isolate showed 100% similarity with H.cajani isolate 252 (Accession No. DQ328693). The phylogenetic relations of H. cajani were evaluated by neighbour joining method. Phylogenetic tree inferred from D2-D3 region of 28S rDNA usingvarious H.cajanipopulations revealedclose relationship ofH. cajaniIARI isolate with H. cajani isolate 252.

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Keywords

Pigeon pea cyst nematode, Heteroderacajani, Molecular characterization, Phylogeny.

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