Callus, as a Prospective Source of Proficient Cells for Determining Phenolic Contents and Antioxidant Potential in Clitoria ternatea L. Ismail Najma1,*, Verma Roop Narayan2, Batra Amla3 1Research Scholar, Plant Biotechnology Laboratory, Department of Botany, University of Rajasthan, Jaipur-302004, Rajasthan, India 2Research Associate, Plant Biotechnology Laboratory, Department of Botany, University of Rajasthan, Jaipur-302004, Rajasthan, India 3Emeritus Scientist, Plant Biotechnology Laboratory, Department of Botany, University of Rajasthan, Jaipur-302004, Rajasthan, India *Corresponding author email id: najmaismail@yahoo.co.in
**dr.rnv26may@gmail
***amlabatra@gmail.com
Abstract A potential medicinal legume Clitoria ternatea L., commonly known as butterfly pea, has been investigated for determining phenolic contents and antioxidant potential. In the present study, callus was used as a prospective source of proficient cells obtained from leaf explants of C. ternatea L. Maximum callus was obtained by Murashige and Skoog, 1962 (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2.0 mg/l). The different in vivo and in vitro (callus) extracts, viz., ethanolic, acetone, petroleum ether, were used for total phenolic contents and antioxidant activity. The total phenol varied from mg/g Distilled Water (dw) in the various extracts was screened for their free-radical scavenging properties using ascorbic acid as standard antioxidant. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging effect of the extracts was determined spectrophotometrically. The highest radical scavenging effect was observed in in vitro acetone extracts with IC50%: 24.4±0.34 μg/ml. The greater amount of phenolic compounds leads to more potent radical scavenging effect as shown by the same extract of C. ternatea L. Top Keywords Clitoria ternatea L, Callus, Ascorbic acid, Phenols, Antioxidant activity. Top |