Detection of spermatozoa and acid phosphatase level in vaginal fluid Dongre A.P., M.D., LL.B., Professor & Head, Shrigiriwar M.B., M.D., Lecturer Department of Forensic Medicine, Shri Y.N. Govt. Medical College, Yavatmal,(M.S.) 445001. Abstract The present study is comprised of 300 females attending Obstetrics & Gynaecology, out patients department in the age group of 15 to 45 years. All studied females were married, parous and their husbands apparently fertile. Separate semen analysis not done. In all 300 female vaginal fluid samples were collected after enquiring details (time & date) about recent vaginal intercourse, douching and contraceptives. The collected samples were kept in refrigerator at 4 degree celsius till the analysis was being done. From each and every sample smears were prepared. The spermatozoa were screened in both native and stained preparations. Quantitative analysis of Acid Phosphatase enzymes in all vaginal fluid samples were done on auto analyser using alpha n,aphthyl phosphate as substrate. The spermatozoa were detected in vaginal fluid upto 24 hours of coitus. Intact sperms were found upto 12 hours and heads of sperm upto 24 hours after coitus. Acid phosphatase enzyme activity was found too high upto 24 hours. It remained persistent upto 36 hours after coitus and declined upto 48 hours after coitus. In all the above subjects, history of douching was not present which probably indicates that it is an unfamiliar practice in this region. Top Key Words Spermatozoa, vaginal fluid, acid phosphatase, sexual assault. Top |