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Transformation of Petunia Protoplasts by Electroporation Choudhary M.L.* Department of Plant Science, Cook College, The State University of New Jensey, USA *Division of Ornamental Crops, I.I.H.R., Hessaraghatta Lake P.O., Bangalore-560089 Abstract Transformation efficiency of electroporated protoplasts of petunia was determined by assaying ?-glucuronidase (GUS) activity. The uptake of foreign DNA by protoplasts of petunia was shown to be dependent on the age of the cell culture from which the protoplasts were isolated and the concentration of DNA used. The maximum GUS activity was discernible when high voltage electric pulse (1000 v/cm) was applied to the solution containing protoplasts and DNA. Transformant calli were selected using kanamycin. Top Keywords Petunia, protoplast, electroporation. Top | |
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