Extraction of high-quality genomic DNA from leaf tissues of marigold Sinha Sweta1, Singh Deepti2,* 1Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur-813 210, Bihar, India 2Department of Horticulture (Vegetable and Floriculture), Bihar Agricultural University, Sabour, Bhagalpur-813 210, Bihar, India *Corresponding author’s e-mail: singh.deepti2008@gmail.com
Online published on 13 March, 2024. Abstract DNA isolation serves as a foundational step in various molecular biology techniques. It provides the starting material for PCR, DNA sequencing and other molecular analyses that are integral to biotechnological applications. High-quality DNA from marigold leaf tissues is generally difficult to extract and purify due to abundance of tannins, alkaloids and polyphenols. Therefore, optimizing DNA extraction protocol from marigold leaves for polymerase chain reaction analysis is crucial to obtaining high-quality DNA suitable for various molecular biology applications. The DNA extracted in the present study is the modified CTAB method which improve overall DNA yield and purity. By carefully optimizing each step of the DNA extraction process without liquid nitrogen, the DNA extracted from marigold tissues is of high quality and suitable for PCR analysis. DNA extracted from marigold using current protocol can be used to study the evolutionary relationships, detection and identification of pathogens, genetic diversity and characterizing genes. Ultimately it will provide valuable information for breeders, researchers and growers to enhance marigold production and develop improved genotypes with desirable traits. Top Keywords DNA, Marigold, PCR, RAPD. Top |