Evaluation of resistance of the house fly, Musca domestica L. to deltamethrin in Tamil Nadu Kavitha K.T.*, Sreekumar C.1, Latha Bhaskaran Ravi, Mangala Gowri A.2 Department of Veterinary Parasitology, Madras Veterinary College, Chennai, 600 007, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu 1Dept. of Wildlife Science, Madras Veterinary College, Chennai, 600 007, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu 2Centralized Instrumentation laboratory, Madras Veterinary College, Chennai, 600 007, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu *Corresponding author. Email: kavitharavi2004@yahoo.com
Online Published on 15 February, 2024. Abstract The house fly, Musca domestica L. is the most common insect pest world-wide, especially in livestock and poultry farms. In the present study, the resistance status of house flies to pyrethroid (deltamethrin) was evaluated using bioassay method and confirmed by allele-specific polymerase chain reaction (AS-PCR). Three field populations were collected from poultry farms at Madhavaram (M isolate), Namakkal (N isolate) and garbage dumps in Chennai (C isolate) in Tamil Nadu. The house flies were tested by residual contact method using three concentrations of deltamethrin (39 ng/cm2, 47 ng/cm2 and 78 ng/cm2 of surface area). The highest mortality (99 %) of house flies was observed at the concentration of 78 ng/cm2. The house flies, when treated with deltamethrin at the double the concentration of 78 ng/cm2, showed 100% mortality in all three populations. AS-PCR was performed using the DNA isolated from the individual house flies (n=30) from each location to detect the presence of knockdown resistance kdr gene (L1014F) frequency. It amplified the 200 bp fragment revealing that only susceptible allele (sus/sus) was present (100%) in all the three populations. The results showed that there was no resistance to deltamethrin in all the three populations of house fly in Tamil Nadu. Top Keywords House fly, Musca domestica, Deltamethrin, Residual contact, AS-PCR, Kdr gene. Top |