IN vitro antibacterial sensitivity of cinnamon and clove oils against gram positive and gram negative bacteria Prajapati J.A.1, Humbal B.R.1, Sadariya K.A.*,3, Bhavasar S.K.4, Thaker A.M.5 1M.V.Sc. Scholar, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and A. H., AAU, Anand-388001, Gujarat 3Assistant Professor, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, AAU, Anand-388001, Gujarat 4Professor & Head, Department of Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, AAU, Anand-388001, Gujarat 5Former Dean & Principal, College of Veterinary Science and Animal Husbandry, AAU, Anand-388001, Gujarat *Corresponding author e-mail: dr_kasadariya@yahoo.co.in
Online published on 8 April, 2021. Abstract The study was planned to evaluate in vitro antibacterial activity of cinnamon and cove oils. Screening of cinnamon and clove oils for antibacterial activity was done by the disc diffusion method against Streptococcus agalactiae (ATCC 13813), Listeria Monocytogenes (ATCC 1911), Staphylococcus aureus (ATCC 6538P), Pseudomonas aeruginosa (ATCC 19154), Escherichia coli (ATCC 10799) and Salmonella typhimurium (ATCC 23564). It was performed using an 18 h culture at 37°C in 10 ml of Muller Hinton Agar (for S. agalactiae 5% defibrinated sheep blood was added). The test suspension was standardized to match 0.5 McFarland turbidity standards. The cinnamon and clove oil were suspended in a solution containing 10% dimethyl sulfoxide and 0.5% tween 80. Under aseptic condition, empty sterilized discs were impregnated with 50 μl of different concentrations (1: 1, 1: 2, 1: 5, 1: 10 and 1: 20) of cinnamon and clove oils and placed on the agar plate surface. Paper disc moistened with vehicle (DMSO plus tween 80) was placed on the seeded petriplate as a vehicle control. Standard disc containing antibacterial drugs (cefotaxime, ampicillin, tetracycline and gentamicin) were used as reference control. The petri plates were incubated at 37°C for 18 h. After the incubation period, the zone of inhibition was measured. The results of the present study revealed that the cinnamon and clove oils showed antibacterial activity. Both gram positive (Staphylococcus aureus, Listeria monocytogenes and Streptococcus agalactiae) and gram negative (Salmonella typhimurium and Escherichia coli) bacteria were sensitive to the cinnamon and clove oils. Pseudomonas aeruginosa was also found sensitive to the clove oil but resistant to cinnamon oil. There was no inhibition in growth of bacteria with the vehicle control. Four antibacterial drugs (cefotaxime, ampicillin, tetracycline and gentamicin) were also tested against all six organisms and were found active against test bacteria. Top Keywords Cinnamon oil, Clove oil, Antibacterial sensitivity, Gram positive, Gram negative bacteria. Top |