Status of amprolium residue in poultry meat sold in Jammu region of Ut of J. & K. Manhas Lenesha1, Pankaj Nrip Kishore1,*, Verma Pawan Kumar1, Rahman Shafiqur2 1Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and Animal Husbandry, R.S. Pura, 181102, India 2Division of Veterinary Pathology, Faculty of Veterinary Science and Animal Husbandry, R.S. Pura, 181102, India *Corresponding author's e-mail: nkp7@ymail.com
Online published on 28 November, 2023. Abstract Amprolium is a common thiamine analogue, anticoccidial agent, which is used indiscriminately in the poultry sector in Jammu region, J&K (UT), primarily for prevention and treatment of coccidia. Therefore, the present study was conducted to detect and quantitate amprolium residue status in chicken tissue. Altogether, 15 samples each, collected from Jammu, Kathua, Reasi and Samba, for amprolium residue estimation. Each sample included liver, kidney, and muscles. The RP HPLC UV method was used to estimate amprolium residue at 263nm. The mobile phase was 0.05M phosphate buffer (pH 5), acetonitrile (30:70; v/v) flow at 1ml/minute, peak Rt at 2.663 min. Recoveries of amprolium from the liver, muscle, and kidney of chicken, spiked at 400 ng/g were 72.156±0.704, 71.029±0.575, 68.316±1.495%, respectively. LOD and LOQ for amprolium were 145 ng/ml and 441 ng/ml, respectively. The value for r2 was 0.999, exhibited good linearity. Out of the samples collected, 66.66% of the liver, 73.33% of kidney, and 86.66% of muscles from district Jammu were positive for residue of amprolium. Likewise, the tissues samples gathered from Kathua, were positive for amprolium residues to the extent of 93.33%, 80%, and 100% of liver, kidney, and muscles, respectively. The tissues of liver, kidney and muscles from Reasi were positive up to 86.66%, 73.33% and 86.66%, respectively. The samples from Samba were positive to the extent of 86.66%, 66.66% and 80% for the residue of amprolium in liver, kidney and muscles respectively. However, none of the tissues collected from all districts exhibited values beyond established international MRL for amprolium residue. Top Keywords Amprolium, RP HPLC UV, LOD, LOQ, MRL. Top |