(18.207.129.175)
Users online: 12119     
Ijournet
Email id
 

Legume Research
Year : 2024, Volume : 47, Issue : 1
First page : ( 147) Last page : ( 151)
Print ISSN : 0250-5371. Online ISSN : 0976-0571.
Article DOI : 10.18805/LR-5210

Identification of Native Root Nodulating Bacteria from Mucuna (Mucuna pruriens L.)

Sharanya B.R.1,*, Gowda A.P. Mallikarjuna2, Muthuraju R.3, Srinivasappa K.N.1, Vasanthi B.G.4, Pushpa K.5

1Department of Horticulture, College of Agriculture, Gandhi Krishi Vigyana Kendra, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India

2Zonal Agricultural Research Station, Gandhi Krishi Vigyana Kendra, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India

3Department of Agricultural Microbiology, Gandhi Krishi Vigyana Kendra, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India

4Dry Land Agriculture Project, Gandhi Krishi Vigyana Kendra, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India

5Department of Agronomy, Gandhi Krishi Vigyana Kendra, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India

*Corresponding Author: B.R. Sharanya, Department of Horticulture, College of Agriculture, Gandhi Krishi Vigyana Kendra, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India, Email: sharanyagowda777@gmail.com

Online Published on 09 February, 2024.

Abstract

Background

Mucuna (Mucuna pruriens L.) is an annual herbaceous climber, grown as a medicinal, green manure, cover and smothering crop. The mucuna seeds contains L-DOPA (L-3, 4 dihydroxy phenylalanine), a non-protein amino acid, extensively used for Parkinson and hypertensive drug. Injudicious application of nitrogenous fertilizers leads to deterioration of soil quality which results into loss of crop yield and quality. The application of microbial inoculant containing efficient native rhizobia enhances the nodular properties, N2-fixation and soil quality. Therefore, Rhizobium strain associated with mucuna was isolated, biochemically characterized and identified. The 16SrRNA sequencing revealed that Sinnorhizobium mililoti, a gram negative symbiotic nitrogen fixing bacteria is present in root nodules of mucuna.

Methods

Root nodules were extracted from mucuna grown at ICAR- Krishi Vigyan Kendra, Doddaballapura Taluk, Bengaluru Rural District, Karnataka, then cultured, screened and characterized in the laboratory. The 16SrRNA sequencing and phylogenetic analysis was done to identify the native rhizobial strain.

Result

Identification of native root nodulating bacteria through 16SrRNA sequencing concluded that Sinnorhizobium mililoti strain associated with the root nodules of Mucuna (Mucuna pruriens L.).

Top

Keywords

16SrRNA, Mucuna, Rhizobium, Sinnorhizobium mililoti.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
722,925,263 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.