Gene Polymorphism Vitamin D receptor FokI in Thalassemia Children in AL-Muthanna Province Waheeb Mohammed Qasim1, Aziz Hanaa Ali1, Alabdali Yasir Adil Jabbar1 1Department of Biology, College of Science, Al Muthanna University, AL-Muthanna, Iraq Online published on 8 August, 2019. Abstract Background and Objective Vitamin D receptor is considered genetic variants that related with vitamin D status. Our study was recorded polymorphism in vitamin D receptor (VDR) FokI in beta thalassemia children. In this study has been shown polymorphism VDR FokI dominant (FF), hybrid(Ff).VDR is includes a beginning codon polymorphism (BCP) that consist of three codon above the course of a second beginning site (ATG). The BCP can be located by the restriction enzyme Fok I, which allele(f)references first of the restriction site ATG is presents, whereas the allele(F) references its missing. Materials and Methods: In this study vitamin D3 levels were evaluated by Enzyme Linked Immunosorbent Assay(Elisa).FokI gene polymorphism were analyzed by using polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP) were estimated in 50 participants children beta thalassemia were distributed to 25 male and 25 female. Results: Patients had significant decrease vitamin D and serum calcium p=0.084 and p=0.751 respectively, alkaline phosphate was recorded p=0.665, potassium= 0.278 and total protein p=0.521. in the male study 84% had VD insufficiency and 16% deficiency, female study 96%had VD insufficiency and 4% deficiency. Conclusion: Vitamin D3 was higher in female more than male and recorded in age category 9–12 years old. VDR FokI gene polymorphism effect VD status, genotype FF, Ff appeared in our study and absence genotype ff. Top Keywords Vitamin D receptor, Gene, polymorphism, FokI, beta-thalassemia. Top |