A study on resveratrol on the Antioxidative and Whitening Cosmeceutical Ingredients Yoon Mi-Yun1, Moon Ji-Sun2, You Seon-Hee3,* 1Department of Cosmetology, Dongnam Health University, 50 Cheoncheon-ro, 74-Gil, Jangan-gu, Suwon-si, Gyeonggi-do, 16328, Republic of Korea 2Department of Beauty Health, Jungwon University, 85, Munmuro, Goesan-eup, Goesan-gun, Chungbuk, 28024, Republic of Korea 3Department of Cosmetology, Dongnam Health University, 50 Cheoncheon-ro, 74-Gil, Jangan-gu, Suwon-si, Gyeonggi-do, 16328, Republic of Korea *Corresponding Author: Seon-Hee You, Department of Cosmetology, Dongnam Health University, 50 Cheoncheon-ro, 74-Gil, Jangan-gu, Suwon-si, Gyeonggi-do, 16328, Republic of Korea, Email: yoush4843@naver.com
Online published on 8 August, 2019. Abstract Objectives Resveratrol is a variety of physiological active substances and is an excellent ingredient in antioxidant, anti-inflammatory and anti-aging action. Therefore, I would like to find out the possibility of adding cosmetics. Method The cytotoxicity test using MTT solution was carried out to check the safety of reverse control. Also DPPH assay was performed to measure antioxidant activity in resveratrol itself, and ROS was measured to measure antioxidant activity in cells. NO production was measured to observe anti-inflammatory action, and tyrosinase activity measurement and melanin production were measured to measure skin whitening activity. Findings we were able to confirm the safety of resveratrol's cytotoxicity. At concentration of 1, 10, 100 μg/mL of Resveratrol, the DPPH radical scavenging activity was observed to show high concentration-dependent activity of free radical scavenging. And In order to check the antioxidant properties within the cell, the ROS (reactive oxygen specifications) using DCF-DA were observed to reduce the effects. The addition of resveratrol 1, 10, 100 μg/mL on RAW 264.7 macrophages stimulated with lipopolysaccharide resulted in NO produce inhibition in concentration dependent and a strong inhibition rate of 53% at 100 μg/mL concentration. Tyrosinase active action was found to inhibited dose dependant. Melanin produce was also prevented by dose dependant. Applications These results suggest that the active oxygen-induced skin inflammation process is delayed as much as possible, so that it can be utilized as a functional cosmetic material having an effect on skin diseases and skin aging. Top Keywords Resveratrol, Antioxidant, DPPH, ROS, Nitric oxide, melanin. Top |