Effects of an aqueous extract from Ammodaucus leucotrichus on calcium oxalate crystallization in vitro Beghalia Mohamed1,2,*, Ghalem Said2, Allali Hocine2, Belouatek Aissa1, Marouf Abdrazek3 1Laboratory of Organic Chemistry, Natural Products and Analysis (COSNA), Department of Chemistry, Faculty of Sciences, University Aboubekr Belkaïd BP 119, 13000 Tlemcen, Algeria. 2Department of Biology, Faculty of Sciences, University of Abdel Hamid Ibn Badis, 27000 Mostaganem, Algeria. 3Department of Biology, Faculty of Sciences, University of Senia, 31000 Oran, Algeria. *Corresponding author: Mohamed Beghalia, E-mail: beghalia_moh@yahoo.fr
Abstract This work focuses on the behavior of in vitro calcium oxalate crystallization. The effects of plant ammodocus on the kinetics of calcium oxalate crystallization were examined. The synthetic urine was prepared immediately before use by mixing with a T-type mixing chamber equal volumes of solutions A and B. Both solutions were prepared by dissolving chemicals of reagent-grade purity in deionised and redistilled water. A: Na2C2O4 (2 m mol/l) B: CaCl22H2O (10 m mol/l). Mixture agitation was maintained to prevent sedimentation. The crystal size development was monitored by polarized microscopy at different time intervals by proceeding as follows: Sample drops were examined every five minutes by polarising optical microscopy. We followed the same experimental procedure for the study of crystallization in the presence of inhibitors. We focussed on crystallisation because it may be the nucleating agent for many stones. As shown with ammodocus, the inhibitory effect was not dose dependent; the best inhibitory concentrations (97.25% and 97.85%) was observed at concentration of [75%] and [100%] mM/L, respectively. The inhibitory effect of ammodocus plant involved growth crystal phases. The growth and agglomeration of calcium oxalate crystals are differently modulated by Ammodocus leucotrichus. Top Keywords Ammodaucus leucotrichus, calcium oxalate, crystallization, in vitro, herbal remedies, rural people, treatment, rheumatism. Top |