In vitro, In vivo antidiabetic activity of Syzygium alternifolium bark and molecular docking studies of isolated compounds against pepck, par, glut2 and glycogen phosphorylase proteins Reddy N.V.L. Suvarchala V.1,*, Raju Ganga M.1, Mamatha M.1, Nawale Sneha Ramesh2, Madhuri Lakshmi M.3 1Department of Pharmacology, Gokaraju Rangaraju College of Pharmacy, Bachupally, Hyderabad-500090, Telangana, India 2Department of Pharmacognosy, Gokaraju Rangaraju College of Pharmacy, Bachupally, Hyderabad-500090, Telangana, India 3Department of Pharmaceutical Chemistry, Gokaraju Rangaraju College of Pharmacy, Bachupally, Hyderabad-500090, Telangana, India *Corresponding author e-mail: suvarchalakiran@gmail.com
Online published on 29 June, 2023. Abstract Prevalence of diabetes mellitus is the rapidly growing around the world, and challenges with the remedies that are currently accessible have motivated scientists to shift their emphasis to naturopathy. The current work sought to identify the Syzygium alternifolium bark's in vitro, in vivo, and in silico antidiabetic potential in streptozotocin and dexamethasone-induced experimental diabetes and to investigate their plausible mechanism of action. There are a number of possible mechanisms for healing the ailment. Inhibiting the intestinal enzymes, such as α-glucosidase and α-amylase, which are in control of intestinal polysaccharide digestion, is the most direct method for preventing monosugars from just being absorbed through the intestinal walls. The phytochemical constituents that are responsible for antidiabetic activity are flavonoids, terpenoids etc., were docked using Schrodinger software and Ramachandran plot analysed for amino acids presence in different regions. MESA was given orally to diabetic rats that were induced using STZ (55 mg/kg bd. wt) at doses of 150 and 300 mg/kg bd. wt for 21 days and 11 days, accordingly. Blood glucose levels were significantly (p<0.05) reduced as a result of MESA treatment, and body weight was also improved. With an IC50 value of 32.57 μg/ml, MESA also triggered a substantial restriction of α-glucosidase enzyme activity. The phytochemicals associated for this strong inhibitory effect in the extract were predicted via docking procedures. Several molecules have exhibited different degrees of inhibition. The study revealed that S. alternifolium may exhibit significant anti-diabetic properties, and this effect may be attributed to the plant's several modes of action, which include antihyperglycemic, postprandial hypoglycemia, hypolipidemic, and actions that stimulate insulin secretion. In order to manage diabetes and its consequences, it may therefore be used as a safer supplemental medicine. Top Keywords Glimepiride, Mesa, Streptozotcion, Dexamethasone, α-glucosidase, Schrodinger software. Top |