Antioxidant, anti-inflammatory, and antimicrobial actions of Paspanguwa: A decoction of traditional five medicinal herbal mixture Madushani H.M.R., Jayawardena P.A.S.N.P., Kadigamuwa C.C.* Department of Chemistry, University of Kelaniya, Sri Lanka *Corresponding author e-mail: cckadigamuwa@kln.ac.lk
Online published on 29 June, 2023. Abstract The name ‘Paspanguwa’ term comes from a combination of five primary herbs Zingiber officinale, Hedyotis corymbosa, Solanum xanthocarpum, Coscinium fenestratum, and Coriandrum sativum. In the present study, water extracts of the individual ingredient and the Paspanguwa decoction were screened for their total soluble phenolic (TPC) & flavonoid (TFC) contents, DPPH radical scavenging activity, and their ability to inhibit protein denaturation. Furthermore, this study focuses on the evaluation of antibacterial and antifungal activities against selected bacterial, and fungal strains. The highest TPC and TFC were seen in C. sativum as 12.76 (± 1.00) μg gallic acid equivalent/g dry weight, and S. xanthocarpum as 778.19 ± 1.40 μg catechin equivalent/g of dry weight respectively. The highest IC50 value for the DPPH assay and reducing power percentage were seen in S. xanthocarpum as 609.7 (± 5.6) μg/mL and C. sativum as 22.95 (± 0.96) respectively. The ability to inhibit protein denaturation varied in the order of Paspanguwa decoction > Z. officinale > C. sativum > C. fenestratum > S. xanthocarpum > H. corymbosa at all three concentrations (625, 1250, and 2500 μg/mL). According to the agar disk diffusion method, the aqueous extracts derived from coriander 0.7 (± 0.1) cm and Paspanguwa mixture 0.7(± 0.1) cm had shown antibacterial potentials only against Staphylococcus aureus bacterial strain. When it comes to antifungal effects, only favourable results were achieved against Candida albicans due to the antifungal activity of ginger 0.8 (± 0.1) cm and Paspanguwa mixture 0.7(± 0.1) cm. These results suggest that Paspanguwa water extract is a good source of antioxidants with TFC and TPC with a higher ability to inhibit protein denaturation, but it is not effective in antimicrobial activities. Top Keywords Paspanguwa, Antibacterial, Antifungal, Anti-inflammatory, Antioxidant. Top |