Evaluation of anticancer potential of methanolic leaf and flower extracts of Rhododendron arboreum Sm in AGS gastric adenocarcinoma cell line Kom Yangchen Dolma, Karthiyayini R.* Department of Botany, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore-641043, Tamil Nadu, India *Corresponding author e-mail: karthiyayini_bot@avinuty.ac.in
Online published on 26 December, 2023. Abstract This study aimed to determine the anticancer potential of methanolic extract of flowers and leaves (RFM, RLM) of Rhododendron arboreum Sm by cytotoxicity and lethality assays. The shrimps in the sample (RFM, RLM) were found to be significantly less toxic in lower and higher concentrations (100, 250, 500, 1000, 1500 μg/ml). Even after 24 h, 1-4 shrimps were observed mortal at the highest concentration, resulted in vigorous cytotoxic activity of methanol extract of flowers, with an IC50 value of 0.541 mg/ml. At the same time, the cytotoxic effects of methanol leaf extract showed comparatively lower cytotoxic effects on AGS cells with IC50 value of 0.841 mg/ml. Low toxicity on normal cells was demonstrated by both the RFM and RLM by the LC50 values, which suggested that both extracts can be considered for anticancer potentials by conducting future experiments. Top Keywords Brine shrimp assay, Flow cytometry, Mortality, MTT, Potassium dichromate and propidium iodide. Top |