Antidiabetic potential of Clitoria ternatea flowers against pancreatic ratio, protein expression of α-amylase, and gene expression of pancreatic glucagon in diabetic rats Lucianus Johan1, Darsono Lusiana1, Widowati Wahyu1,*, Rusmana Djaja1, Tiono Hartini1, Onggowidjaja Philips1, Kusuma Hanna Sari Widya2, Nindya Faradhina Salfa2 1Faculty of Medicine, Maranatha Christian University, Bandung40164, West Java, Indonesia 2Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung40163, West Java, Indonesia *Corresponding author e-mail: wahyu_w60@yahoo.com
Online Published on 14 March, 2024. Abstract Diabetes Mellitus (DM) is a hormonal disorder characterized by decreased insulin levels in the blood. The use of synthetic drugs for DM treatment is associated with various adverse effects that can harm patients, leading to an increased demand for herbal remedies. Clitoria ternatea flower extract (CTE) is believed to be antidiabetic because of its antioxidant activity. This study was conducted to determine the potential of CTE as an antidiabetic in rats that have been induced by streptozotocin (STZ) and a high-fat diet (HFD). In vivo testing was conducted on Sprague Dawley rats induced by STZ and HFD, administering CTE at doses of 200, 400, and 800 mg/kg BW orally for 28 days. The expression of pancreas protein αamylase using immuno-histochemistry (IHC) and pancreatic glucagon was assessed using the qRT-PCR method. Results indicated that CTE was able to restore the pancreas state close to normal, decrease the expression of the α-amylase protein, and reduce glucagon gene expression in the DM rats model. The concentration of 800 mg/kg BW of CTE exhibited the highest efficacy. This study showed that CTE holds promise as a treatment for diabetes mellitus. Top Keywords Clitoria ternatea, Diabetes mellitus, Gene expression, Immunohistochemistry. Top |