Progressive Agriculture
Year : 2023, Volume : 23, Issue : 1
First page : ( 53) Last page : ( 62)
Print ISSN : 0972-6152. Online ISSN : 0976-4615.
Article DOI : 10.5958/0976-4615.2023.00007.8

Microbial sensitivity test of zinc and silver nano particles

Kumar Lalit^*

College of Applied Education and Health Sciences, Meerut

^*Corresponding Author Email- lalitkumar0402@gmail.com

Online published on 20 May, 2023.

Abstract

In the present investigation, of Zinc Oxide (ZnO) and Silver nanoparticles (NP), and their application on pathogenic bacteria was investigated. ZnO NP were synthesized by chemical reduction method usingstarch as capping agent and silver NP was prepared by green synthesis process from AgNO3 solutionthrough the extract of Citrus sinensis (sweet lime). From the analysis of UV-VIS spectroscopy theformation of nanoparticles was confirmed. Antibacterial assay of synthesized ZnO and silver NP wascarried out both in liquid and solid growth medium against four pathogens (Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and streptococcus pneumonia). The bacterial growth was monitored by measuring the optical density (OD) of culture solution and estimation of colony forming units (CFU) on solid medium. The effect of NP on the level of gene expression in E. coli was also examined. When NP was administered in the liquid E.coli culture, considerable enhancement of the enzymatic activity of expressed β-glucosidase was observed. Further the physical interaction between bovine a-lactalbuminprotein and NP was monitored by tryptophan fluorescence and circular dichroism spectroscopy. Whenthe interaction was monitored by tryptophan florescence spectroscopy, a drastic conformational changeof the protein was observed. The interaction was also monitored by circular dichroism spectroscopy. Itrevealed that on binding, nanoparticles caused substantial change in the secondary structure of theprotein which indeed in complete agreement with tertiary conformational change monitored by florescence spectroscopy.

Top

Keywords

Nanoparticles (ZnO and Silver), Antimicrobial effect, β-glucosidase, A- lactalbumin,tryptophan florescence, Circular dichroism spectroscopy protein-nanoparticles conjugate.

Top