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Molecular analysis of Leucinodes orbonalis Guen. populations within Tamil Nadu using lepidopteran specific random primers Geetharajalakshmi S., Subramanian S., Shanmugasundaram P. S., Mohankumar S.* Department of Plant Molecular Biology and Biotechnology, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore-3, Tamil Nadu, India. *Corresponding Author
Abstract Genomic DNA of ten different populations belonging to different eggplant growing regions were analyzed for RAPD profiles, for understanding the intraspecific variation among them. Twenty-five “lepidopteran specific random primers” were used in this analysis, which generated a lotal of 279 markers revealing an average of 10–12 markers per primer in each population. The primers generated polymorphic markers (249), monomorphic markers (35) with a percentage of polymorphism (87.6). The per cent of polymorphism ranged from 46.15–100 for different primers. The results are discussed in relation to the genetic relationship among the ten populations. Top Key words Genetic diversity, Leucinodes orbonalis molecular markers, RAPD. Top | |
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