Development and application of an analytical method for salivary inorganic anion quantification for the diagnosis of systemic and oral diseases Park Jung-Eun1, Jang Jong-Hwa2,* 1Department of Preventive and Social Dentistry, School of Dentistry, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul, 130–701, Republic of Korea 2Department of Dental Hygiene, Hanseo University, 360 Daegok-Ri, Haemi-Myun, Seosan-Si, Chungnam, 356–706, Republic of Korea *Corresponding Author E-mail: Je3207@naver.com, jhjang@hanseo.ac.kr
Online published on 21 August, 2018. Abstract Background/Objectives The purpose of this research was to determine a method to analyze several salivary anions simultaneously using HPLC (IC) and to analyze the anions in actual saliva samples. Methods/Statistical analysis Chromatographic determination was performed using a Dionex Ion column Pac AG 11-HC 50mm×4mm. The column parameters were as follows: mobile phase, isocratic elution with 120 mmol/L NaOH; flow rate, 1, 000 μL/min; separation temperature, 35°C. Findings The linearity of the sample's linear range was 0.9902–0.9989, LOD was 0.78–1.16μM, and LOQ was 2.36–3.49 μM. The recovery test showed a recovery rate of 95.63–107.77%, and RSD of 3.37–11.48%, verifying the method of analysis. Improvements/Applications The method of analysis used in this study can appropriately quantify anions in saliva. Hence, it could be used in biomarker studies. Top Keywords Saliva, Inorganic anion, Ion, Biomarker, HPLC. Top |