Quick and efficient method for callus culture from stem disc tissue of garlic (Allium sativum L.) Priyanka, Majumdar Rita Singh*, Cynthia Muteba Ndiba Department of Biotechnology, School of Engineering and Technology, Sharda University, Greater Noida, 201306, India *Corresponding Author E-mail: rita.singh@sharda.ac.in
Online published on 21 August, 2018. Abstract The present study discusses a quick, efficient and improved procedure for the establishment and maintenance of garlic callus culture. The culture was induced from stem discs meristematic tissue of garlic cloves on Murashigue and Skoog (MS) medium supplemented with different concentrations and combinations of auxins and cytokinins. The study was further prolonged to analyze the potentials of the established calluses to be maintained in laboratory conditions for a longer period of time. Quick and efficiently productive callus formation was attained averagely only after 6 days for old harvest (OH) and 20 days for new harvest (NH) explants with a medium supplemented with 2, 4-D. Callus growing in the modified MS medium having 2.5 and 3 mg/l of 2, 4-D gave the best responses showing a callus induction percentage of 82% and 77% for NH and 95% and 87% for OH explants respectively. Callus initiation was also good with comparatively better morphological growth in the media supplemented with 0.25mg/l 2, 4-D + 0.4mg/l kin than in media only with 2, 4-D giving a percentage callusing response of 80% in NH and 85% in OH explants but approximately doubled the time delay. Maintenance of callus for longer duration was best observed in the media supplemented with 2, 4-D and Kinetin at the combined concentrations of 0.25mg/l and 0.4 mg/l respectively with an increase in callus size over time. Top Keywords Callus culture, Stem disc, Allium sativum, tissue culture. Top |