Cytotoxic potential of 4-Hydroxypentan-2-Oneextracted from Jacaranda mimosifolia on colorectal cancer cells Ragunathan Adhithya, Ravi Lokesh, Krishna Kannabiran* Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India *Corresponding Author E-mail: kkb@vit.ac.in
Online published on 24 August, 2018. Abstract The incidence of colorectal cancer is comparatively higher in the industrialized countries. It has been estimated that the incidence, of colorectal cancer, would be increased significantly in the next twenty-fiveyearsworldwide. Therefore researchers are looking for an effective anti-colorectal cancer drug from natural sources. The aim of the present study was to isolate and to identify anticancer cytotoxic phytochemical compound from Jacaranda mimosifolia and to study its mechanism of action. The cytotoxic activity of the crude extracts (acetone, petroleum ether, and chloroform) prepared from J.mimosifolia on HCT-15 cell line was evaluated by MTT assay. Among the extracts, acetone extract showed the IC50 value of 600μg/ml on the HCT-15 cells. The acetone extract was purified by silica gel column chromatography, and the lead compound obtained was characterized byFT-IR, 13C-NMR, and GC-MS. The structure of the pure compound was identified as 4-hydroxypentan-2-one and it showed the IC50 value of 100μg/ml against HCT-15 cell line. The mechanism of action of 4hydroxypentan-2-one on HCT-15 cancer cells drug target proteins was assessed by In Silicomolecular docking analysis using the bioinformatics tool AutoDock. The pure compound 4-hydroxypentan-2-one inhibited the K-Ras protein and showed the least binding energy of-5.22 Kcal/mol with the inhibition constant of 148.89 μMand 6 hydrogen bonds were formed during the interaction. Top Keywords Jacaranda mimosifolia, HCT-15, Colorectal cancer, 4-hydroxypentane-2-one, AutoDock. Top |