Distribution of antibiotic resistance genes in Acinetobacter species and its genotypic prevalence Nahar N.1,*, Adib M.2, Rashid R. B.1,** 1Computational Chemistry and Bioinformatics Laboratory Department of Pharmacy, State University of Bangladesh, Dhaka-1205, Bangladesh 2Department of Pharmaceutical Chemistry, University of Dhaka, Dhaka, 1000, Bangladesh *Corresponding Author E-mail: nusratnahar17@gmail.com
**ridwan@sub.edu.bd
Online published on 24 August, 2018. Abstract The present study analyzed total nineteen Acinetobacter species for antibiotic resistance genes responsible for multidrug-resistant (MDR) phenotype through in silico tools. In silico PCR amplification identified sixteen antibiotic resistance genes in Acinetobacter species among the twenty-six resistance genes studied here. Insertion element ISABA1 (57.89%) was present upstream of the blaOXA. Adenylyltransferase (aadA1) and acetyltransferase (aacC1 and aacC4) genes were detected in 42.11%, 31.58% and 5.26% isolates, respectively. Only one isolate (5.26%) harboured the phosphoryltransferase, aphA6 and streptomycin resistance, strA gene. Fluoroquinolone resistance gene, parC was detected in 78.95% (n=15) of the isolates while tetracycline resistance determinants, tetA and tetB were found in only two isolates. The ampC beta-lactamase and blaTEM-1 genes were present in 68.42% and 15.79% isolates, respectively. Only one isolate expressed blaVEB-1 and blaPERgene while no metallo beta-lactamase (blaIMP, blaVIM, blaGIM)genes were found. The blaOXA-23 gene was detected in 26.32% of the isolates. The most prevalent OXA type was blaOXA-51 (78.95%). Genotyping was performed by in silico pulsed field gel electrophoresis (PFGE) that grouped nineteen isolates into six genotypes. Genotype 4 was more resistant since it harboured 14 antibiotic resistance genes. Genotype 2 and 5 contained no antibiotic resistance genes while genotype 6 harboured only fluoroquinolone resistance gene, parC. This study helps to assess the distribution of these resistance genes based on genotypes. It also helps to select antibiotic for the treatment of Acinetobacter infections and improve the clinical outcomes with severe Acinetobacter infections. Top Keywords Acinetobacter, Antibiotic resistance genes, Genotype, In silico, PCR, Multidrug-resistant, Pulsed field gel electrophoresis. Top |