Assessment of Cinamic acid and Costus roots extract against MDR-K. pneumoniae isolated from patients of COVID-19 Hussein Mohammed S.1, Al-Qaysi Al-Moghira K.2, Meani Safaa Abed Latef Al2, Ahmed Mohammed Mukhles2,*, Abed Nihad Shahadha2, Ibrahim Mohammed Obaid3 1Department of Biology, College of Science, University of Anbar, Anbar, Iraq. 2Department of Biotechnology, College of Science, University of Anbar, Anbar, Iraq 3Ministry of Health, Anbar health departmentIraq. *Corresponding Author E-mail: moh.mukhles@uoanbar.edu.iq
Online Published on 06 March, 2023. Abstract The emergence of resistant Klebsiella pneumoniae associated with COVID-19 demonstrate a primary challenge for the antimicrobial therapy of infectious diseases and increases the incidence of mortality and morbidity. "K. pneumoniae isolated from COVID-19 patient's sputum with ratio (100%). All K. pneumoniae clinical isolates had 100% resistance to ceftriaxone, piperacillin (80%), cefepime (60%), amikacin (40%), and meropenem =levofloxccin (20%). Bacterial isolates gave positive result for mCIM with ratio was 100%, also all isolates produced diversity of β- lactamases at a rate of 100% by using spectrometry β-lactamase assay. Costunolide (38.3 %), Rutin (15.33%), Pentadecanoic acid (6.54%), Oliec acid (4.77%), and Caproic acid (3.22%) considered as major compounds in Saussurea costus were identified by GC-Mass spectrometry. The β-lactamase produced by K. pneumoniae were inhibited by Saussurea costus with a strong statistical significance at P- value: <0.01, while cinamic acid donot effected on β-lactamase activity. Top Keywords Saussurea costus, Antibacterial activity, Klebsiella pneumoniae, Costunolide. Top |