Effect of amlodipine in proliferative retinopathy: Biochemical estimation and zebrafish (Danio rerio) Modeling Nanda Ashirbad1,3, Sai Aparna2, Sahoo Rudra Narayan1,3, Pramanik Arunima1, Satpathy Bhargab4, Mallick Subrata1, Das Debajyoti1, Patri Manorama2,* 1School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Orissa751003, India 2Neurobiology Laboratory Department of Zoology, School of Life Sciences, Ravenshaw University, College Square, Cuttack, Orissa753003, India 3Centurion University of Technology and Management, Orissa, India. 4Research Associate II Hetero Biopharm Unit III, Hyderabad, Telangana *Corresponding Author E-mail: mpatri@ravenshawuniversity.ac.in
Online published on 14 June, 2022. Abstract Proliferative retinopathy is described as a progressive neovascularization in the inner surface of the retina or vitreous, which subsequently threatens vision by causing retinal detachment or vitreous hemorrhage. Reactive oxygen species generation and its related vasoproliferative factor up regulation play a major role in progression of the disease. The present study focuses on Carr induced reactive species generation with its related vasoproliferative factor up regulation and ameliorative activity of AML. Biochemical estimation of catalase, lipid peroxidation, hypoxia inducing factor (HIF-1a) and guanyl-s-transferase (GST) were done. AML treated group showed significant increase in catalase enzyme, guanyl-s-transferase and significantly reduced the lipid peroxidation. Western blotting assay showed a significant decrease in HIF-1a in AML treated group. The progression of PR alters the endogenous cell signalling thus causing havoc in retinal cells. Therefore, it could be hypothesized that use of AML can be a new detection method for therapeutic strategies to prevent blindness in PR. Top Keywords Amlodipine, Proliferative retinopathy, Biochemical estimation, Hypoxia. Top |