Research Journal of Pharmacy and Technology
Year : 2022, Volume : 15, Issue : 3
First page : ( 1282) Last page : ( 1286)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.52711/0974-360X.2022.00214

RP-HPLC method for simultaneous estimation of tobramycin and dexamethasone in eye drop

Mittha Jyoti N.^*, Kalshetti Mallinath S.

D.S.T.S. Mandal's College of Pharmacy, Solapur

^*Corresponding Author E-mail: jyoti.mittha68@gmail.com

Online published on 14 June, 2022.

Abstract

RP-HPLC method has been developed and validated for simultaneous estimation of Tobramycin and Dexamethasone in eye drop. The chromatographic separation was carried out isocratically on Agilent Zorbax SB Aqueous C-18 column (250x4.6mm, 5µm) using a 0.1% Formic Acid Water: Acetonitrile (60:40% v/v) as mobile phase at flow rate of 1ml/min and detected by using photo diode array detector at 210nm. The retention times were 1.9 and 4.2 min for tobramycin and dexamethasone respectively. Linearity was obtained in the range of 240–360µg/ml and 80–120µg/ml for tobramycin and dexamethasone respectively. The developed method was validated according to ICH guidelines for specificity, linearity, range, accuracy, precision, system suitability parameters. The proposed method was found to be specific, accurate and precise. This method can be employed for routine analysis of simultaneous estimation of tobramycin and dexamethasone in eye drop.

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Keywords

Tobramycin, Dexamethasone, RP-HPLC, Simultaneous Estimation, Eye Drop.

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