Development and validation of rp-hplc method for the estimation of alogliptin in api and tablet formulation Ingle Suyash*, Tegeli Varsha, Birajdar Avinash, Nangare Gajanand D.S.T.S. Mandal's College of Pharmacy, Solapur - 413004, Maharashtra, India *Corresponding Author E-mail: suyashingle18@gmail.com
Online Published on 08 June, 2022. Abstract The analytical method was developed and validated for determination of Alogliptin in bulk and pharmaceutical dosage forms by High performance liquid chromatography. The separation was carried out on Zorbax SB-Aq (250 x4.6mm,5µ ID) column. The mobile phase consists of 0.1% TFA Water: ACN in the ratio 62:38 at flow rate 1ml/min with diode array detector wavelength at 290nm.The column temperature was adjusted at 30° ± 0.5°C with injection volume 10µl.The retention time of Alogliptin was 3.06min. The linearity of the calibration curve was linear over the concentration range 25–75µg/ml (r2=1). The validation was carried out as per ICH guidelines. The development of method was easy, rapid, linear, precise, accurate and consistent. Top Keywords Alogliptin, RP-HPLC, Validation, Method development, 290nm and diode array detector. Top |