Diabetic wound healing in IL-1β expression by Nano Herbal of Zanthoxylum acanthopodium and Rhodomyrtus tomentosa Manurung Risma D.1, Ilyas Syafruddin1,*, Hutahaean Salomo1, rosidah Rosidah2, Situmorang Putri C.1 1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia, 20155 2Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia, 20155 *Corresponding Author E-mail: syafruddin6@usu.ac.id
Online published on 20 February, 2023. Abstract Wounds of diabetic patients cause damage to skin tissue for a long time. Increased glucose levels affect angiogenesis which triggers the wound to heal. This study was to determine IL-1β expression in diabetic wound healing after being given nano herbal of Zanthoxylum acanthopodium and Rhodomyrtus tomentosa. This study consisted of 4 treatment groups. K1: negative control, K2: MEBO, K3: Nano herbal of andaliman fruits (100mg/kg BW), and K4: Nano herbal of haramonting leaves (100mg/kg BW) on days 0, 4, 8, 12, and the 16th. The tissue was prepared on paraffin blocks and given Immunohistochemistry staining. Results showed that there was a significant difference between all groups (P <0.001) in IL-1β expression. Histologically in wound healing, the granulation tissue of blood vessels is dominated by extensive fibroblasts and collagen. The epithelium has covered the wound that occurred on the skin. This suggests that some of the compounds contained in the nano herbal haramonting and andaliman can stimulate cell division and growth to form scar tissue. In conclusion, epithelium covered the wound in MEBO treatment, showing that the histology of the skin tissue was almost the same as that of nano herbal andaliman and haramonting. Therefore Nano herbal andaliman and haramonting can be developed into herbs that can be used to treat wounds in diabetics. Top Keywords Diabetes mellitus, IL-1β, Rhodomyrtus tomentosa, Wound, Zanthoxylum acanthopodium. Top |