Evaluation of In-vitro antioxidant and In-vivo antidepressant properties of flax seed extract (Linum usitatisimum, L.) Shaida Bushra1, Singh Karuna2, Rastogi Mayuri3,* 1Assistant Professor, Department of Food Technology, Jamia Hamdard University, New Delhi, India 2Professor, Department of Nutrition and Dietetics, Sharda School of Allied Health Sciences, Sharda University, Greater Noida, Uttar Pradesh, India 3Assistant Professor, Department of Nutrition and Dietetics, Sharda School of Allied Health Sciences, Sharda University, Greater Noida, Uttar Pradesh, India *Corresponding Author E-mail: mayuri.rastogi@sharda.ac.in
Online Published on 5 February, 2024. Abstract Flax seed (Linum usitatisimum, L.) extract have been used for the treatment of numerous diseases. Despite of its health benefits, its action was not much studied on neurotic disorders like depression and anxiety. Thus the objective of the presentresearch work was to explorein-vitro antioxidant activity and in-vivo anti-depressive and anti-anxiety property of seed extract. The extraction method were optimized to yield the highest contents of biochemical compounds and antioxidant activities, further extract was evaluated for phytochemicals. The antioxidant potential of the extract was assessedusing DPPH.In-vivostudy was done by dividing mice into 5groups and each group have six mice thus the total number of mice for the study is 30, initially they were trained to consume 2% sucrose solution for 3 weeks.The first group was normal control, the remaining four were exposed to stress and were fed with either: 10ml water per kg p.o, imipramine (antidepressive drug)15 mg/kg body weight, diazepam(anti-anxiety drug) 25mg/kg body weight and flaxseed extract in dosages of (40 mg, 60 mg, 80mg per kg body weight) for the time period of 30 days.Results for in-vivo activity indicate that flaxseed extract of 80 mg/kg body weight and imipramine groups had significantly increased sucrose consumption, decreased duration of immobility during Forced Swim Test (FST) and tail suspension test (TST) thus indicating significant antidepressive activity of the extract. Similar results were seen for anti-anxiety effect, evaluation was done using photoactometer and elevated plus maze method. Top Keywords Linum usitatisimum L., Phytochemicals, Folin-Cioucalteu reagent, Forced swim test, Tail suspension test, Photoactometer. Top |