Platelet derived growth factor expression after administration of okra fruit extract on diabetic wistar rats Luthfi Muhammad1,*, Tantiana1, Rachmawati Aisyah Ekasari2, Razak Fathilah Binti Abdul3 1Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya - Indonesia 2Undergraduate Student of Faculty of Dental Medicine, Universitas Airlangga, Surabaya - Indonesia 3Department of Oral and Craniofacial Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia *Corresponding Author E-mail: m.luthfi@fkg.unair.ac.id, mluthfi.fkgunair@gmail.com
Online Published on 5 February, 2024. Abstract Background Increased AGEs in diabetics promote a protracted inflammatory response and macrophage malfunction in growth factor release, one of which being Platelet Derived Growth Factor (PDGF). The disruption of proliferation and angiogenesis is influenced by a reduction in PDGF expression. Okra (Abelmoschus esculentus) fruit extract possesses antioxidant, anti-inflammatory, and anti-diabetic properties. This extract aids in the inflammatory process, macrophage proliferation, and immunomodulatory macrophage roles in the production of growth factors. Purpose The goal of this study was to observed if giving an okra fruit extract to a diabetic Wistar rat will improve the expression of PDGF in the wound healing process following tooth extraction. Methods 24 Wistar rats were divided into 2 groups: Control Group (Streptozotocin-induced), and Treatment Group (Streptozotocin-induced and treated with okra fruit extract 250mg/kgB Woral sonde once a day after extraction). Extraction was performed on the lower jaw left incisor. Four rats from each group were sacrificed on day 3, 5, and 7 after the extraction. To evaluate the number of PDGF expression, the socket on the left lower jaw was cut open and immunohistochemistry was performed. Results There was a significant difference between the number of PDGF expressions on the treatment group on day 3 (10.92245±0.80934), day 5 (15.71875±1.31646) day 7(17.71875±1.24506) with control group on day 3(3.71875±1.65004), 5(7.94791± 0.51637), and 7(7.89062±0.61740). Conclusion On diabetic Wistar rats, okra fruit extract modulates the increase in PDGF expression in wounds after tooth extraction. Top Keywords Okra, Platelet-Derived Growth Factor (PDGF), Wound Healing, Diabetes, Medicine. Top |