HPTLC quantification of α-Glucosidase inhibitor mangiferin in hydro-alcoholic extract of Salacia species and antidiabetic poly-herbal formulation Sanandia Jalpa1,*, Vadalia Jigna2, Thakur Mousmi1, Sheth Navin2 1Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360005, Gujarat, India 2Graduate School of Pharmacy, Gujarat Technological University, Ahmedabad-382424, Gujarat, India *Corresponding Author E-mail: jalpa.sanandia@gmail.com
Online Published on 5 February, 2024. Abstract Salacia oblonga Wall. and Salacia reticulata Wight. (Family Celastraceae) are commonly known as Saptarangi and used in Ayurvedic medicine as potent antidiabetic agent. It is a woody climber, mainly habitat in Sri Lanka and Southern India. A large number of chemical constituents such as salacinol, neosalacinol, kotalanol, neokotalanol, and mangiferin were isolated from stem and root of saptarangi which shows various therapeutics activities. Chemical constituent presents in both species of Salacia are working as α-glucosidase inhibitor for diabetes management. Among them mangiferin is a plant natural polyphenol of C-glycosylxanthone structure and found in many plant species. Many chromatography techniques like LC-MS, HPLC are available to quantify mangiferin but no proper data available for simple, rapid, precise, economic HPTLC method. So, HPTLC method was developed with mobile phase ethyl acetate: formic acid: water (4:0.5:0.5 v/v/v) which confirmed the presence of mangiferin at 0.38±0.01 Rf value observed under 254nm. Mangiferin was present in 1.02% in Salacia oblonga root extract, 0.94% in Salacia reticulata stem extract and 0.42% in polyherbal formulation. The validated HPTLC method will be useful in standardization of different Ayurvedic formulations using mangiferin as a marker. Top Keywords HPTLC, Mangiferin, Salacia oblonga, Salacia reticulata, Saptarangi. Top |