Reverse Phase Liquid Chromatography for Cetrimide Determination in Pure and Pharmaceutical Preparations Alkayar Ziad T I1, Ismail Ahmed A.1, Al-Ani Nagham Muthanna Jassim2, Alekseevna Fedoseenko Anastasia3, Saeed Ahmed Mahdi1, Al-Amir Haider Mahmoud Nasser2, Yaseen Mahmood Shakoori2, Tawfiq Bashaar Mohammed2, Jassem Zena M.4, Hassan Shaimaa Ahmad5,*, Ala Maryam O. Abd1 1Department of Chemistry, College of Education for Pure Science, University of Diyala, Iraq 2The State Enterprise for Drugs Industries and Medical Appliances, Iraq 3Emperor Alexander I St. Petersburg State Transport University, Russia 4Ministry of Trade Iraq- grain board-quality Control Department, Iraq 5College of Remote Sensing and Geophysics, Al Karkh University of Science, Baghdad, Iraq *Corresponding Author E-mail: dr.shaimaa_altaee@kus.edu.iq
Online Published on 07 February, 2024. Abstract An effective, duplicable, and quick method to determine the purity and formulating form of Cetrimide (CE) have studied and modified utilizing the Reverse Phase High Performance Liquid Chromatography (RP-HPLC). The method was based on the separation of the CE using a reverse phase HPLC Shimadzu LC–20 A, Japan, and Phenomenex C18 column (15 × 4.6mm, 5.0μm). The process was carried out by using the mobile phase ammonium acetate solution 0.1M (AA), methanol (MeOH), and the methyl cyanide in the ratio of (15:60:25 V/V/V), a flow rate of 1.0mL/min, at pH = 3, with 10μL sample loop and the UV - spectrophotometry detector was set at 280nm. However, for the optimum conditions the mobile phase composition, wavelength, pH, and flow rate were studied. The calibration carve has obtained in the range of 10-1000mg/L and the correlation coefficient is 0.9985. The average recovery of the standard drug was 97.56%. The LOD was 5mg/L and LOQ is 16.5mg/L for CE. The process was used successfully to determine the CE in the pharmaceutical forms with the average recovery of 95.61 and 96.02%. Top Keywords Cetrimide, Spectrophotometry, RP - HPLC, Pharmaceutical, Ammonium acetate. Top |