Development and Validation of a New HPLC Method for the Estimation of Sulfamoxole in Bulk and Tablet Formulations Moparthy Kamala Karuna1, Ratnakaram Venkata Nadh2,*, Gorumutchu Giri Prasad3 1Department of Chemistry, Government College for Women (A), Guntur - 522001, India 2Industrial Chemical Product Development and Analysis Centre, Department of Chemistry, GITAM School of Science, GITAM Deemed to be University – Bengaluru Campus, Karnataka – 561203, India 3Department of Chemistry, A.G & S.G.S College, Vuyyuru, Krishna, District - 521165, India *Corresponding Author E-mail: doctornadh@yahoo.co.in
Online Published on 07 February, 2024. Abstract To quantify the sulfamoxole content in bulk as well as tablet formulation, a new isocratic RP-HPLC method was developed and then validated. Kromasil C18 column with a dimension of 250 X 4.6mm was used which was filled with a particle size of 5μ. A mixture containing CH3OH, CH3CN and H2O in the volume ratio of 55, 30 and 15 was used as a mobile phase at room temperature with an optimized flow rate of 1ml/min. UV detector was set at 241nm for sulfamoxole determination. The run time of the current method is ten minutes. Accomplished the forced degradation studies to understand the stability indicating nature of the current method. As per the current ICH guides (Q2R1), validation of the method was conducted. Top Keywords Sulfamoxole, RP-HPLC, Method development, Validation. Top |