Spectrophotometric Multi-Component Quantitation of Rosiglitazone, Glibenclamide and Metformin HCl in Pharmaceutical Dosages Form by using Cramer’s Matrix Baghel Uttam Singh1,2,*, Sharma Abhay2, Gautam Harshit2, Mukim Mohammad3, Singh Deeksha4, Dave Shuchi5, Srivastava B.6, Singh Bhawani7 1Gurukul Pharmacy College, Ranpur-325003, Kota, Rajasthan, India 2Department of Pharmacy, University of Kota, Kota-324005, Rajasthan, India 3Kota College of Pharmacy, Ranpur-325003, Kota, Rajasthan, India 4E. S. I. Hospital, Kota-324005, Rajasthan, India 5Guru Ramdas Khalsa Institute of Science and Technology (Pharmacy), Barela, Kukrikheda, Jabalpur-483001, Madhya Pradesh, India 6School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, India 7Department of Pure and Applied Chemistry, University of Kota, Kota-324005, Rajasthan, India *Corresponding Author E-mail: drusb1985@yahoo.com
Online Published on 07 February, 2024. Abstract Objective This article illustrates the development of a simple, precise and accurate spectrophotometric analysis method for the determination of Rosiglitazone, Glibenclamide and Metformin hydrochloride in their pharmaceutical preparations by using Cramer’s matrix. Method The absorbance of the sample solution was measured at 315.6nm and 229.2nm for the estimation of rosiglitazone and glibenclamide, respectively and at 237.0nm for estimation of metformin hydrochloride by Shimadzu double beam UV-visible spectrophotometer. Results The result of the analysis for method was tested and validated for various parameters according to ICH guidelines and found satisfactory. The method was successfully applied for the determination of these drugs in their tablet dosage forms. Conclusion Simultaneous estimation of active ingredients in multicomponent pharmaceutical products normally requires the use of chromatographic separation techniques, like thin layer chromatography, high performance thin layer chromatography, high performance liquid chromatography or gas chromatography etc., followed by their quantitation. But this article illustrates direct spectroscopic estimation of multicomponent formulation by using Cramer’s matrix. Top Keywords UV Spectrophotometer, Rosiglitazone, Glibenclamide, Metformin hydrochloride, Cramer’s matrix, Validation. Top |