Whey preparation as an adjuvant to minimise the Intestinal inflammatory status in mucositis rat model Reddy Sudarshan1,*, Rao Gayathri M2,**, Marathe Aradhana3,***, Kamath Saritha4,**** 1Associate Professor, Department of Pharmacology, SSPM Medical College, MUHS, Nashik 2Associate Professor, Department of Biochemistry, Kasturba Medical College, MangaloreManipal Academy of Higher Education, Manipal, India 3Department of Biochemistry, Kasturba Medical College, MangaloreManipal Academy of Higher Education, Manipal, India 4Associate Professor-Senior Scale/Head, Dept. of MLT, Manipal College of Health Professions, Manipal Academy of Higher Education, Manipal *Corresponding Author E-mail: sudhubio@gmail.com
**gayathri.rao@manipal.edu
***aradhana.marathe@manipal.edu
****sarith.kamath@manipal.edu
Online Published on 07 February, 2024. Abstract To study the effect of Whey Preparation on intestinal mucositis, model developed by administration of single dose of etoposide (60mg/Kg body wt., i.p). The experimental procedure included Adult albino rats (10±1 week old) of Wistar strain (n=30, 6/group). Freshly prepared whey preparation-(100 and 200mg/Kg/body wt.) was administered by orogastric gavage- Pre, both pre and post etoposide injection. Effectiveness of whey preparation was determined by estimating the biochemical parameters including intestinal Tumor Necrosis Factor-α, Interleukin-6, sodium potassium ATPase, inflammatory markers- Cyclooxygenase and Myeloperoxidase along with Nitric Oxide and reduced Glutathione with and without intervention after 72 hrs. of etoposide injection. There was a significant increase in the Tumor Necrosis Factor –α, Interleukin-6 levels and extent of apoptosis in etoposide controls. Etoposide administration caused a significant decreased the activity of Na+-K+ ATPase and reduced glutathione levels in intestinal tissue. However, in the post treatment groups, Tumor Necrosis Factor–α, Interleukin-6 as well as extent of apoptosis were reverted to normal after whey preparation administration.Whey Preparation gavage in both the study groups- pre and both pre and post etoposide exposure showed that intervention was able to preserve/ prevent changes in the biochemical activity. The results support the WP potentials to reduce the deleterious effects of etoposide on normal cells, which leads to mucositis. Top Keywords Mucositis, IL-6, TNF alpha, Whey preparation, Etoposide. Top |