ZENITH International Journal of Multidisciplinary Research
Year : 2019, Volume : 9, Issue : 1
First page : ( 252) Last page : ( 257)
Online ISSN : 2231-5780.

Invitro studies of ocimum sanctum using various plant growth regulators

Deepa Mrs. K.P.

Assistant Professor, Department of Botany, Bishop Heber College (Autonomous), Tiruchirappalli, Tamilnadu, India. E-mail: deepacck@gmail.com

Online published on 23 January, 2019.

Abstract

Biotechnological implications can challenge the shortages produced by human interventions on the natural resources. The micro propagation using known chemicals and growth regulators can produce metabolites of various interests. In the present study the growth promotion studies of Ocimum sanctum, in in-vitro conditions using different expalnts were studied. The study revealed 1.0 mg/L BAP of Cytokinin produce proliferation from the nodal explant compared to other combinations. Also 0.1 mg/L IAA and 1.0 mg/L BAP shows good proliferation of shoot tip below 30 days. The study proved to be a good tool in the optimization of growth medium for the plants and also for the plants coming under Lamiaceae. The calli formed from the leaf and internode explant where brown to yellow in colour and this takes 20–30 days for initiation. By comparing the studies of length of proliferated shoot, number of shoots and days for proliferation, this study shows the nodal explants were the best explats for invitro micro propagation of Ocimum sanctum.

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Keywords

Micro propagation, in-vitro studies, growth regulators, explants, plant regeneration.

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