Pathology, serotyping and phylogeny of foot and mouth disease viruses circulated in the cattle of Sirajganj district, Bangladesh Hoor-E-Jannat M.1, Islam M.S.1, Bari A.S.M.1, Khan M.A.H.N.A.1 1Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh *Corresponding author: e-mail: hadi.khan@bau.edu.bd
Abstract Foot-and-mouth disease (FMD) is endemic in cattle in Bangladesh. There was increased morbidity and mortality of cattle in Bangladesh due to FMD. This study was carried out during July, 2015 to investigate the cause of mortality and molecular characterization of the FMD viruses (FMDVs). Out of 50 cattle investigated, 22 cattle were infected with FMDV, of which 4 young calves and 5 adult cattle were died. Gross and histopathological investigation of dead animals revealed cardiomyonecrosis and severe respiratory distress; these lesions may be related to the death of infected young and adult cattle. Viral RNA was extracted from the oral lesions of acutely infected cattle and used in uniplex RT-PCR, which successfully detected FMD viruses (430bp). The multiplex RT-PCR (732bp) detected FMDV serotype A in the cattle of Sirajganj district, Bangladesh. The VP1, 2A and 2B genes of FMDV serotype A was amplified (732bp) and sequenced. There was mutation in 21 to 22 points in the VP1 gene (306bp). The mutation lacked in 2A and 2B genes. Phylogenetic analysis carried out using sequence of VP1 gene revealed that the FMDV serotype A was belonging to genotype VII of Asian topotype. It needs to examine large number of samples to identify other existing serotype and topotype of FMDVs affecting cattle at Sirajganj district. The level of mutation as seen in the VP1 gene may be related to increased pathogenicity of the viruses and the genetic variation noted may be a valuable tool to design future vaccine candidate. Top Keywords FMD virus serotype A, Sequencing, Lpro and VP1 genes, Mutation, Phylogenesis. Top |