Histopathological classification and immunohistochemical characterization of canine mammary tumours Raval S.H.5,*, Joshi D.V.5, Parmar R.S.5, Patel B.J.5, Patel J.G.5, Patel V.B.1,5, Ghodasara D.J.2,5, Chaudhary P.S.3,5, Kalaria V.A.4,5, Charavala A.H.5 5Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India; 1Animal Disease Investigation Office, Government of Gujarat, Vadodara, Gujrat; 2Department of Veterinary Pathology, College of Veterinary Science & A.H., AAU, Anand, Gujrat; 3Chief Veterinary Surgeon, Surat Panjara Pole Trust, Nandini Veterinary Hospital, Surat, Gujarat; 4Department of Veterinary Pathology, College of Veterinary Science & A.H., Junagadh Agricultural University, Junagadh, Gujarat, India *Corresponding author: e-mail: samirraval81@gmail.com
Abstract The present study was carried out to investigate breed wise and age wise prevalence of canine mammary tumours (CMTs) along with histopathological and immunohistochemical characterization. A total of 97 CMTs samples were collected from different parts of Gujarat, India over period of three years (April 2014-March 2017). The mean age of the dogs, involvement of breeds and mammary glands were recorded. The mean±SD age of all affected dogs was 7.5±2.8 years. Among 12 breeds affected, most cases (n=77) of CMTs were recorded in four breeds viz., Pomeranian, Mongrel, Germen Shepard and Labrador. Caudal abdominal and inguinal mammary glands were frequently showed neoplastic growth. Among histological characterization of 97 CMTs, 16(16.49%) were non neoplastic proliferative/dysplastic lesions, 11 (11.34%) were benign neoplasms and 70 (72.16%) were malignant tumours. Out of 70 malignant neoplasms, epithelial neoplasms, special type epithelial neoplasms, mesenchymal neoplasms and carcinosarcomas were 53(54.64%), 6(6.19%), 8(8.25%), and 3(3.09%), respectively. Further, mixed carcinoma, tubulopapillary carcinoma, solid carcinoma, carcinoma and malignant myoepithelioma, tubular carcinoma and ductal carcinoma were frequently noted. Immunohistochemistry was found to be a powerful tool to classify the CMTs. In the present study, smooth muscle actin (SMA) helped in diagnosing complex adenoma, complex carcinoma, malignant myoepithelioma and fibrosarcoma. In complex adenoma, complex carcinoma and malignant myoepithelioma, myoepithelial cells showed strong SMA immunoreactivity, although stromal myofibroblasts and vascular smooth muscle cells also immunostained. Top Keywords Canine mammary tumours, Histopathological classification, Immunohistochemistry. Top |