Antifungal potential of native Trichoderma isolates against Pythium aphanidermatum causing Chilli damping-off Pandey Manisha*, Ahmad Shafaat1, John Suchit A., Khan Kunwar Zeeshan1 Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Prayagraj-211 007 (U.P.) 1Department of Plant Pathology, Sam Higginbottom University of Agriculture, Technology & Sciences, Prayagraj-211 007 (U.P.) *pandeymaai@gmail.com
Online published on 20 July, 2019. Abstract Efficacy of 23 Trichoderma isolates was tested for their ability to reduce the growth of Pythium aphanidermatum causing damping-off of chilli. Studies revealed that locally isolated Trichoderma harzianum showed the highest inhibition (98.1%) followed by T. asperellum (88.8%), T. hamatum (86.6%), T. longibrachiatum (85.2%) and T. koningiopsis (84.3%) and other Trichoderma spp. against the growth of P. aphanidermatum. Highly potential five isolates of Trichoderma were selected for pot culture evaluation for germination %, pre and post-emergence damping-off and growth parameter of chilli seedlings. Among five Trichoderma isolates, seed treatment with T. harzianum at 2×108 CFU ml−1 recorded highest germination (93.7%), shoot length (10.03 cm) and root length (05.65 cm) and was also better in reducing the pre and post emergence damping-off (84.3% and 85.0%, respectively) followed by T. asperellum and rest of the treatments. The antimicrobial volatile compounds isolated from T. harzianum were 6 pentyl-α-Pyrone, Heneicosane, Harzianic acid and 1, 2 Benzene dicarboxylic acid. Top Keywords Capsicum annuum, Pythium aphanidermatum, GC-MS, Trichoderma.. Top |