Isolation and identification of Trichoderma spp. through ITS-PCR from Chick pea growing areas of Andhra Pradesh Nagamani P., Bhagat Someshwar1, Viswanath K.2, Biswas M.K. Department of Plant Protection, Palli Siksha Bavana, Viswava Bharati, Bolpur-731 235 (West Bengal) 1NRRI-Central Rainfed Upland Rice Research Station, Hazaribagh-825 301 (Jarkhand) 2Regional Agricultural Research Station, Tirupati-517 502 (Andhra Pradesh), India manipath28@gmail.com
Online published on 7 April, 2020. Abstract Trichoderma isolates isolated from different chick pea growing areas of Andhra Pradesh region were studied ITS-PCR. Collection of 16 isolates was further characterized into two main clades by internal transcribed spacer (ITS) sequence analysis. Consequently, a reliable phylogenetic tree was constructed containing isolates belonging to two groups. Group 1, the T. aspergillum, T. longibrachiatum Trichoderma spp. and Group II was outgroup includes T.viride, T. asperillum and T. asperelloides complex. The phylogenetic analysis showed many solitary clades of T.asperellum, which indicates that more diversity among the T. asperellum. Further, this analysis showed less bootstrap values indicates instability of the clade. Top Keywords Cicer arietinum, ITS-PCR, Molecular characterization, Trichoderma spp. Top |