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Annals of Plant Protection Sciences
Year : 2020, Volume : 28, Issue : 1
First page : ( 52) Last page : ( 59)
Print ISSN : 0971-3573. Online ISSN : 0974-0163.
Article DOI : 10.5958/0974-0163.2020.00013.0

Potential Bacillus strains having plant growth promoting and volatile activity against pathogens of Pea (Pisum sativum L.)

Pandey Neetu*, Sharma O.P.

Mewar University, Chittorgarh-312 001 (Rajasthan), India

*Neetupandey85@gmail.com

Online published on 7 April, 2020.

Abstract

Fifty four bacterial isolates were isolated from rhizosphere of Pea growing in different regions of India and screened for potential plant growth promoting (PGP) and antagonistic activities through volatile compound secretions. Based on morphology and biochemical test, seventeen bacterial isolates were identified as Bacillus sp. and confirmed by HiBacillus Identification Kit (Himedia laboratories). A total of seventeen isolates (43.7%) produced IAA more than 60.0 μg mL−1, 31.2% solubilize phosphate and 100% isolateswereproduced ammonia. Bacillus isolates were secreted 64.7%, 47%, 58.8% and 5.8% of cellulase, protease, amylase and lipase, respectively. The Bacillus isolates UKMI 19 and UKMI 32 showed maximum growth inhibition against Rhizoctonia solani and Fusarium oxysporum. UKMI 32 and UKMI 19 suggest that they may be exploited as a potential bioinoculant agent in pea field.

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Keywords

Bacillus strains, PGP, Rhizoctonia solani, Volatile activity.

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