Annals of Plant Protection Sciences
Year : 2020, Volume : 28, Issue : 1
First page : ( 67) Last page : ( 71)
Print ISSN : 0971-3573. Online ISSN : 0974-0163.
Article DOI : 10.5958/0974-0163.2020.00016.6

Appearance of Cotton leaf curl disease begomovirus avirulent strains associated with a lower disease incidence in Delhi

Duhan Manisha, Gupta Subhankar, Bhattacharyya U.K., Palchoudhury S., Biswas K.K.*

Advanced Centre for Plant Virology, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi-110 012

*drkkbiswas@yahoo.co.in

Online published on 7 April, 2020.

Abstract

A survey of cotton leaf curl disease (CLCuD) in cotton fields of ICAR-IARI, New Delhi was made to study the disease incidence and determine the causal agents of CLCuD-begomovirus species or variants. The CLCuD incidence was estimated to 20.7% in the year 2018 and 26.3% in 2019 and it was observed lower than in the previous years. Five CLCuD-begomovirus isolates collected from infected fields were characterized based on sequence analysis of complete CP gene. The present isolates shared 99–100% nt identity among them, and 71–91% identity among the other CLCuD begomoviruses. Based on phylogenetic analysis, three isolates were members of Cotton leaf curl Multan virus-Rajasthan (CLCuMuV-Ra) and two were Cotton leaf curl Kokhran virus-Burewala (CLCuKoV) strains. Of five CLCuD isolates, four were detected as recombinants showing a common break point at 759-52nt (P=1.768 × 10⁻¹). Thus, the occurrence of present lower degree of CLCuD may be due to the appearance of putative recombinant and avirulent CLCuMuV and CLCuKoV stains.

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Keywords

Gossypium hersutum, CLCuD incidence, Phylogeny, Recombination.

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