Antioxidant Enzymes as Markers of Human Periapical Lesions: Are Lipid Peroxides Responsible? Krishan Shalu*, Prof & Head, Aggarwal Anurag, Sr. Lect. Deptt Of Conservative Dentistry and Endodontics, Swami Devi Dyal Hospital & Dental College, Golpura, BarwalaDistt.panchkula * Room No 6, Deptt Of Conservative Dentistry & Endodontics, Swami Devi Dyal Hospital & Dental College,Golpura, Barwala, Distt.Panchkula Email:shalukg2007@rediffmail.com Abstract The westernization of the world with rise in habits e.g. alcohol and cigarettes and the influence of radiations and chemicals is giving rise to diseases namely, Hypertension, Cancer, Arthritis etc.The woes are added on further by unhealthy eating habits causing dental afflictions of various kinds.Periapical lesions are frequently occurring dental diseases which are basically a host-immune response.In biochemical terms, the Reactive Oxygen Species (ROS) produced by inflammatory cells cause injury to host cell leading to inflammatory reaction.The most common injury is caused by lipid peroxidation of Unsaturated Fatty Acids in our body.To combat these deleterious effects of ROS, our body has certain cytoprotective enzymes such as: Catalase, Glutathione peroxidase, and Superoxide dismutase.Hence, we evaluated the presence of the antioxidant enzymes and the Lipid peroxides in periapical tissue. For this we took two groups: gingival group as control and the periapical group as the experimental group.The patient's history and a written consent was obtained.Then periapical surgery was performed in periapical lesions and in case of gingival group, gingivectomy was done.The tissue sample was washed thoroughly in chilled saline and the sample was stored at −80C in ultra deep freezer. Later a tissue homogenate was prepared from each sample and the levels of Catalase, Glutathione peroxidase, Superoxide dismutase and Lipid peroxides were estimated in each of them. The result showed that the levels of the antioxidant enzymes in the experimental group were low as compared to the control group and the levels of Lipid peroxides were higher in periapical group. Thus we concluded that the fall in level of antioxidant enzymes can be considered as markers of inflammation and the rise in the level of Lipid peroxides may be considered responsible for the pathogenesis of periapical lesions. Top Keywords Glutathione peroxidase, Catalase, Superoxide dismutase, periapical lesions. Top |