Decolorization of dyes with immobilized laccase Thakare Rakesh U. Department of Microbiology, Yashwantrao Chawhan College, Lakhandur, Dist.- Bhandara. Online published on 17 October, 2020. Abstract Present investigation was undertaken on production of laccase enzyme by Pleurotus pulmonarius, Bartalinia sp. (MM101), Rhizopus oryzae and Trametes hirsute and its immobilization. Decolorization of dyes with the help of immobilized laccase enzyme was also studied. Reactive Red M8B, Reactive Green 19 and effluent showed decolorization to the extent of 30 % in case of Reactive Red M8B and effluent, while 55 % in Reactive Green 19, after immobilization of the crude enzyme with 100 ml of 2 % Sodium Alginate. There was significant variation in the extent of decolorization of various dues; however, the difference due to the organisms, from which the enzyme was isolated, was statistically non-significant. Top Keywords Immobilization, Laccase enzyme, Pleurotus pulmonarius, Bartalinia sp. MM101, Rhizopus oryzae, Trametes hirsute, Reactive Red M8B, Reactive Green 19. Top |