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Indian Journal of Animal Nutrition
Year : 2023, Volume : 40, Issue : 2
First page : ( 129) Last page : ( 135)
Print ISSN : 0970-3209. Online ISSN : 2231-6744.
Article DOI : 10.5958/2231-6744.2023.00017.8

Determination of available phosphorus in compounded cattle feed using citrate buffer by ICP-OES

Gupta S.K.*, Pal B., Jitiya M., Chawla R., Nair R.

CALF Laboratory, National Dairy Development Board (NDDB), Post Box No. 40, NDDB Campus, Anand, Gujarat-388 001

*Correspondence: guptask@nddb.coop

Online published on 22 August, 2023.

Abstract

A study was carried out to develop the test method for the determination of available phosphorus in compounded cattle feed manufactured. There are a few methods of determination of the available phosphorus, however these are lengthy, complex and cumbersome. Therefore, it was required to develop a fast and robust method of determination of available phosphorus. Cattle feed samples from different sources were extracted using citrate buffer of pH 3 and then available phosphorus was analyzed using ICP-OES after microwave digestion of the extract to determine available phosphorus. The study yielded promising and consistent results of available phosphorus in compounded cattle feed in terms of recovery of the analyte ranging from 96.5 to 109%. The study showed that the proposed test method can be used by a laboratory to determine available phosphorus in compounded cattle feed with accuracy and precision.

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Keywords

Available Phosphorus, Citrate Buffer, Feed, ICP-OES, Phosphorus.

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