Effects of prostasomes on functional parameters of fresh and cryopreservedthawed spermatozoa of crossbred Karan Fries (KF) bulls Kumar Amit1,*, Pandita Sujata2, Laxmi N. Anand3, Bhakat Mukesh2, Mohanty T.K.2 Department of Animal Physiology, National Dairy Research Institute, Karnal-132 001, Haryana, India 1Faculty of Veterinary Animal Science, IAS, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India 2National Dairy Research Institute, Karnal-132 001, Haryana, India 3Poultry Research Station, Hyderabad-500 030, Telangana, India *Corresponding author's e-mail: dramitndri24@gmail.com
Online published on 15 October, 2019. Abstract Prostasomes are extracellular vesicles that fuse with sperms thereby improving its functional parameters. Present study aimed to isolate and characterise prostasomes from semen of KF bulls, and to investigate prostasomes effects on functional parameters of KF bull spermatozoa. Isolated prostasomes were characterized with respect to the binding of FITC-conjugated CD 26 antibodies, as well as protein, cholesterol and phospholipids content. Subsequently, effects of prostasomes supplementation (1mg/ml) were investigated on ROS production, Ca2+ signalling, mitochondrial membrane potential and acrosome integrityof fresh and cryopreserved-thawed spermatozoa. Isolated prostasomes were immunostained positively. Prostasomes showed higher proportion of both protein and cholesterol as compared to phospholipids. When sperm samples were supplemented with prostasomes, ROS production decreased, while all other functional parameters improved. Top Keywords Bull, Prostasomes, Sperm functional parameters. Top |