Identification of single nucleotide polymorphism in GnRHR gene in Murrah bulls Reen J.K.*, Ramesha K.P., Preeti, Revanasiddu D., Ahirwar M. Genetics Laboratory Dairy Production Section, ICAR-National Dairy Research Institute, Southern Regional Station, Adugodi, Bengaluru-560 030, Karnataka, India *Corresponding author's e-mail: reenjagaish119@gmail.com
Online published on 15 October, 2019. Abstract The present study was undertaken with the objectives of molecular characterization and detection of genetic polymorphism in the GnRHR gene in Murrah bulls. Blood samples were collected from 109 Murrah bulls maintained at three different organized semen stations viz., Centralized Semen Collection Centre of Livestock Breeding and Training Centre, Dharwad; Nandini Sperm Station and State Livestock Breeding and Training Centre, Hessarghatta, Bengaluru, Karnataka, India. Genomic DNA was isolated from the blood samples within 24 hours of collection. Mutations were screened using Polymerase Chain Reaction-Single Stranded Conformational Polymorphism (PCR-SSCP) technique followed by Sanger Sequencing. PCR-SSCPmethod revealedsimilar band pattern within Murrah bulls. To confirmmonomorphism in the studied population with respect to GnRHR gene, duplicate samples from each primer fragment were custom sequenced. PCR-SSCP and sequence analysis revealed monomorphism within the studied population that is coding region as well as exon-intron boundaries of GnRHR gene is highly conserved among Murrah bulls. However, a total of 28 Single Nucleotide variations (SNV's) have been found when compared with Bos Taurus reference sequence for GnRHR gene. Top Keywords Gonadotropin releasing hormone receptor, Monomorphism, Murrah bulls, PCR-SSCP. Top |