Analysis of certain blood biochemical parameters in relation to oxidative stress in chronic mitral valve insufficiency of dogs with heart failure Indhu M.S., Sesh P.S.L.1, Loganathasamy K., Jeyaraja K.2, Padmanath K., Pandiyan V.* Department of Veterinary Biochemistry, Madras Veterinary College, TamilNadu Veterinary and Animal Sciences University, Chennai-600 007, Tamil Nadu, India 1Department of Veterinary Physiology and Biochemistry, Veterinary College and Research Institute, Ramayanpatti, Tirunelveli-627 001, Tamil Nadu, India 2Department of Veterinary Clinical Medicine, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India *Corresponding author's e-mail: mypands@rediffmail.com
Online published on 15 October, 2019. Abstract Chronic mitral valve insufficiency (CMVI) is the most common acquired heart disease in dogs. In heart failure, the cellular oxygenation and metabolism are affected, which leads to the production of free radicals. Free radicals damage DNA, lipid and protein molecules in cells. In the present experiment, blood samples were collected from CMVI dogs with heart failure and were compared with the results obtained from healthy dogs. A significant increase in the levels of xanthine oxidase, AST, LDH and CK and decrease in the activity of catalase were noticed in CMVI dogs when compared to healthy dogs, which revealed overall cardiac and skeletal muscle damage in CMVI dogs. Results of biochemical parameters revealed an increase in urea level and decrease in sodium, potassium and calcium levels in CMVI dogs as compared to control dogs, all of which indicate cardiac damage in dogs. Study on hematological parameters revealed a significant decrease in Hb, PCV, RBC and platelet counts and an increase in total WBC counts and percentage of neutrophils, decrease in percentage of the lymphocyte and monocyte in CMVI dogs than control. These results indicate secondary phenomenon to heart failure. The present research data indicates the usefulness of these biomarkers in the diagnosis and prognosis of CMVI with heart failure in dogs. Top Keywords Cardiac enzymes, Chronic mitral valve insufficiency, Heart failure, Oxidative stress, Xanthine oxidase. Top |